| 质粒名称: | pLamp1-mScarlet3-H (aka mYongHong) |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000754 |
| 产品用途: | Expresses Lamp1-mScarlet3-H in mammalian cells |
| 存储实验室: | Zhifei Fu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | Lamp1, mScarlet3-H |
| 物种来源: | H.sapiens, Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A highly stable monomeric red fluorescent protein for advanced microscopy
Xiong H., Chang Q., Ding J., Wang S., Zhang W., Li Y., Wu Y., Lin P., Yang C., Liu M., Fang G., Yang Y., Xie J., Qi D., Jiang T., Fu W., Hu F., Chen Y., Yue R., Li Y., Cui Y., Li M., Fan S., Yang Y., Xu Y., Li D., Zhang F., Zhao H., Wu C., Zheng Q., Piatkevich KD., Fu Z.
Nat. Methods 2025 1–11 (2025)
|
pLamp1-mScarlet3-H (aka mYongHong)
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pLamp1-mScarlet3-H (aka mYongHong)载体质粒基本信息
pLamp1-mScarlet3-H (aka mYongHong)质粒图谱载体图谱和pLamp1-mScarlet3-H (aka mYongHong)载体序列质粒序列多克隆位点信息
载体图谱.png)
pLamp1-mScarlet3-H (aka mYongHong)质粒载体简介
pLamp1-mScarlet3-H (aka mYongHong)质粒序列载体序列
LOCUS pLamp1-mScarlet3-H (aka mYongHong) 5857 bp DNA circular SYN 30-DEC-2024
DEFINITION TSN20241013-0571-00010_754_20241014-BAN4_E03_E03_i20.1.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5857)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5857
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 157..745
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
enhancer 920..1223
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1224..1427
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 1456..2676
/codon_start=1
/product="rat lysosome-associated membrane glycoprotein 1"
/label=LAMP1
/translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
SHAGYQTI"
regulatory 2686..2695
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 2692..3381
/codon_start=1
/label=mScarlet3-H
/translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKH
ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
TGGSGGS"
polyA_signal 3504..3625
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3632..4087)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4114..4218
/gene="bla"
/label=AmpR promoter
promoter 4220..4577
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4428..4563
/label=SV40 ori
/note="SV40 origin of replication"
CDS 4612..5406
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 5638..5685
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
1 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
61 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
121 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
181 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
241 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
301 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
361 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
421 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
481 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
541 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
601 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
661 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
721 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
781 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
841 ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca ttagttcata
901 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
961 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
1021 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
1081 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
1141 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
1201 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
1261 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
1321 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
1381 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
1441 gtcagatccg ctagcatggc ggccccgggc gcccggcggc cgctgctcct gttgctgctg
1501 gcaggccttg cacacagcgc cccagcactg ttcgaggtga aagacaacaa cggcacagcg
1561 tgtataatgg ccagcttctc tgcctccttt ctgaccacct atgaggctgg acatgtttct
1621 aaggtctcga atatgaccct gccagcctct gcagaagtcc tgaagaatag cagctcttgt
1681 ggtgaaaaga atgcttctga gcccaccctc gcaatcacct ttggagaagg atatttactg
1741 aaactcacct tcacaaaaaa cacaacacgt tacagtgtcc agcacatgta tttcacatat
1801 aacctgtcag acacacaatt ctttcccaat gccagctcca aagggcccga cactgtggat
1861 tccacaactg acatcaaggc agacatcaac aaaacatacc gatgtgtcag cgacatcagg
1921 gtctacatga agaatgtgac cattgtgctc tgggacgcta ctatccaggc ctacctgccg
1981 agtagcaact tcagcaagga agagacacgc tgcccacagg atcaaccttc cccaactact
2041 gggccaccca gcccctcacc accacttgtg cccacaaacc ccagtgtgtc caagtacaat
2101 gtgactggtg acaatggaac ctgcctgctg gcctctatgg cactgcaact caacatcacc
2161 tacatgaaga aggacaacac gactgtgacc agagcattca acatcaaccc aagtgacaaa
2221 tatagtggga cttgcggtgc ccagttggtg accctgaagg tggggaacaa gagcagagtc
2281 ctggagctgc agtttgggat gaatgccact tctagcctgt ttttcctgca aggagttcag
2341 ttgaacatga ctcttcctga tgccatagag cccacgttca gcacctccaa ctattccctg
2401 aaagctcttc aggccagtgt cggcaactca tacaagtgca acagtgagga gcacatcttt
2461 gtcagcaagg cgctcgccct caatgtcttc agcgtgcaag tccaggcttt cagggtagaa
2521 agtgacaggt ttgggtctgt ggaagagtgt gtacaggacg gtaacaacat gctgatcccc
2581 attgctgtgg gcggggccct ggcagggctg gtcctcatcg tcctcatcgc ctacctcatc
2641 ggcaggaaga ggagtcacgc gggctatcag accatcccac cggtcgccac catggatagc
2701 accgaggcag tgatcaagga gttcatgcgg ttcaaggtgc acatggaggg ctccatgaac
2761 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
2821 gccaagctga gggtgaccaa gggtggcccc ctgcccttct cctgggacat cctgtcccct
2881 cagttcatgt acggctccag ggccttcacg aagcaccccg ccgacatccc cgactactgg
2941 aagcagtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
3001 gccgtgtccg tggcccagga cacctccctg gaggacggca ccctgatcta caaggtgaag
3061 ctccgcggca ccaacttccc tcctgacggc cccgtaatgc agaagaagac aatgggctgg
3121 gaagcatcca ccgagcggtt gtaccccgag gacgtcgtgc tgaagggcga cattaagcat
3181 gccctgcgcc tgaaggacgg cggccggtac ctggcggact tcaagaccac ctacagggcc
3241 aagaagcccg tgcagatgcc cggcgccttc aacatcgacc gcaagttgga catcacatcc
3301 cacaacgagg actacaccgt ggtggaacag tacgaacgct ccgtggcccg ccactccacc
3361 ggcggctccg gtggctccta aagcggccgc gactctagat cataatcagc cataccacat
3421 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
3481 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
3541 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
3601 tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa
3661 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
3721 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
3781 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
3841 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
3901 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
3961 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
4021 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
4081 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
4141 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
4201 tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
4261 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
4321 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
4381 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
4441 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
4501 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
4561 gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa
4621 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
4681 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
4741 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
4801 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
4861 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
4921 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
4981 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
5041 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
5101 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
5161 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
5221 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
5281 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
5341 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
5401 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
5461 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
5521 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
5581 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
5641 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc
5701 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
5761 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
5821 ccaacgtcgg ggcggcaggc cctgccatag cctcagg
//
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